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In principle, the cell membrane-bound antibody is the only fraction that is available for pretargeting with a non-internalizing mude. Notably, the antibodies previously used successfully for pretargeted imaging either accumulate to the tumour at exceptionally high concentrations such nuve 5B1 targeting the carbohydrate Vilma models nude CA In this work, the known internalization of the selected mAbs brings an additional challenge for the pretargeted system together with the residual unbound antibody remaining in the blood flow at the time of tracer injection. Despite these complications, we could localize the tumour in the PET images, showing that pretargeted imaging strategies could be extended to the imaging of a wider selection of antibodies with careful tailoring of the radiotracer pharmacokinetics.

Cells were harvested and passaged weekly using 0. Cetuximab was also treated analogously before use, although the formulation did not include any possibly interfering ingredients. TCO-mAb was purified with size-exclusion chromatography PD10 and with spin-column centrifugation three times Ultracel Scheme S1 and its precursors were synthesized as previously described [ 24 ]. Animal experiments All animal experiments were carried out in compliance with protocol approved by the Institutional Animal Care and Use Committee of Memorial Sloan Kettering Cancer Center and followed National Institutes of Health guidelines for animal welfare.

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Crude radiolabelling yields and radiochemical purities ITLC: The higher molecular weight and more hydrophobic nature of DFO appears to have a slight impact on the number of moieties conjugated, as well as the immunoreactivity of the mAbs compared to TCO. Determination of optimal imaging time point for the pretargeting experiments with 89Zr-labelled antibodies DFO-cetuximab and DFO-trastuzumab were radiolabelled with 89Zr to visualize their biodistribution in A and BT xenograft-bearing mice in order to determine the optimal time point i. Web of Science Citations: Heterogeneous expression of A33 in colorectal cancer: A comprehensive characterization of cell cultures and xenografts derived from a human verrucous penile carcinoma.

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